![]() Here we describe deployment of this technology in Homalodisca vitripennis Germar (glassy-winged sharpshooter, GWSS). All that is required for direct mutagenesis is a high-quality draft genome, a means to introduce the Cas9 protein and single-guide RNAs (sgRNAs) to the germline, and the ability to perform genetic crosses. ![]() This will facilitate novel genetic control strategies.ĬRISPR/Cas9 technology provides the means to direct mutagenesis to a specific site in a genome its application to insect pests brings genetic control strategies to the forefront in insect species where genetic manipulations have either proven elusive or not been attempted. Our data show that GWSS can be easily developed as a genetic model system for the Hemiptera, enabling the study of traits that contribute to the success of invasive pests and vectors of plant pathogens. We used amplicon sequencing to examine the extent of off-target mutagenesis in adults arising from injected eggs, which was found to be negligible or non-existent. Our analysis of wing phenotype revealed an unexpected discovery of the participation of pteridine pigments in wing and wing-vein coloration, indicating a role for these pigments beyond eye color. Through pair matings, we obtained 100% transmission of w and cn alleles to the G3 generation and also established that both genes are located on autosomes. We use a novel and simple approach of embryo microinjection in situ on the host plant and obtain high frequency mutagenesis, in excess of 55%, of the cinnabar and white eye pigmentation loci. We report the establishment of genetic analysis in the glassy-winged sharpshooter (GWSS), Homalodisca vitripennis, which is a significant leafhopper pest of agriculture in California. 1980 10:467–500.CRISPR/Cas9 technology enables the extension of genetic techniques into insect pests previously refractory to genetic analysis. The metabolism of aromatic amino acids concerned in the crosslinking of insect cuticle. Detection and transmission of a gramnegative, xylem-limited bacterium in sharpshooters from a citrus grove in Florida. Colonization of the sharpshooter vectors, Oncometopia nigricans and Homalodisca coagulata, by xylem-limited bacteria. Dispersion of Homalodisca coagulata (Cicadellidae: Homoptera), a vector of Xylella fastidiosa, into vineyards in southern California. Age determination and the red pigment in the wings of the glassy-winged sharpshooter. San Diego: California Department of Agriculture 2008. Proceedings of the 2008 Pierce's Disease Research Symposium. vitripennis was composed almost entirely of older insects.īextine B, Lauziere I. Finally, field collected insects from several Central Texas vineyards were successfully analyzed for age determination suggesting that the annually reported influx of H. The impact of three environmental conditions on these readings was also investigated and found to have little effect on the age determination, and could be easily accounted for. vitripennis was developed using laboratory-reared insects of known ages varying from 1 to 60 days. In this study, a standard curve for the age determination of H. vitripennis can be determined by calculating the amount of red pigment found in the wings by analyzing high resolution wing photographs with image analysis software. These pigments are believed to be pheomelanin and eumelanin, respectively. vitripennis lifespan, the red pigment darkens and eventually becomes brown/black in color. This insect is the main vector of the plant-pathogenic bacterium Xylella fastidiosa Wells (Xanthomonadales: Xanthomonadaceae), the causal agent of Pierce's disease of grapevines. A red pigment is contained in the wing veins of the glassy-winged sharpshooter, Homalodisca vitripennis (Hemiptera: Cicadellidae).
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